abstract：:Local application of K+ via micropressure-ejection, coupled with in vivo electrochemical detection, was used to study stimulated release from monoaminergic nerve terminals in the striatum of anesthetized rats and mice. K+-evoked releases were reversible, reproducible, and dose-dependent. In contrast, releases of elect...

journal_title：Journal of neurochemistry

authors： Gerhardt GA,Rose GM,Hoffer BJ

更新日期：1986-03-01 00:00:00

abstract：:beta-nerve growth factor (NGF) was modified by biotinylation via carboxyl group substitution (C-bio-NGF) using biotin hydrazide and the coupling reagent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, under reaction conditions that yielded an average of 3 biotin additions per NGF subunit. NGF was also biotinylated thr...

journal_title：Journal of neurochemistry

authors： Rosenberg MB,Hawrot E,Breakefield XO

更新日期：1986-02-01 00:00:00

abstract：:The immediate acyl chain donor for fatty acid esterification of proteolipid protein (PLP) was identified in an in vitro system. Rat brain total membranes, after removal of crude nuclear and mitochondrial fractions, were incubated with radioactive acyl donors, extracted with chloroform/methanol, and analyzed by sodium ...

journal_title：Journal of neurochemistry

authors： Bizzozero OA,Lees MB

更新日期：1986-02-01 00:00:00

abstract：:We have demonstrated by indirect immunofluorescence the cellular localization of a monoclonal antibody (mAb 224-58), produced after immunization of a mouse with human central nervous system (CNS) myelin. Serologically, mAb 224-58 was found to be specific for 3'-sulfomonogalactosylglycolipids, namely 3'-sulfogalactosyl...

journal_title：Journal of neurochemistry

authors： Guerci A,Monge M,Baron-Van Evercooren A,Lubetzki C,Dancea S,Boutry JM,Goujet-Zalc C,Zalc B

更新日期：1986-02-01 00:00:00

abstract：:The incorporation of radioactive precursors into gangliosides and other glycolipids, glycoproteins, and total lipids has been studied in rat pheochromocytoma PC12 cells. Starting with the same PC12 cell pool, cultures displaying different degrees of neuritic expression in response to nerve growth factor (NGF) and comb...

journal_title：Journal of neurochemistry

authors： Katoh-Semba R,Skaper SD,Varon S

更新日期：1986-02-01 00:00:00

abstract：:The binding of a series of glycosylated beta-galactosidases to a fraction rich in synaptic membrane of bovine brain was examined. beta-galactosidase modified with p-aminophenyl beta-D-galactopyranoside (beta-D-Gal beta-gal) was found the most effective in binding to synaptic membrane, followed by that modified with be...

journal_title：Journal of neurochemistry

authors： Naoi M,Nagatsu T

更新日期：1986-02-01 00:00:00

abstract：:Sympathetic denervation of the iris muscle produces increases in both the breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) and in muscle contraction in response to norepinephrine (NE). To shed more light on the biochemical basis underlying this supersensitivity we investigated: the effects of NE on PIP2 break...

journal_title：Journal of neurochemistry

authors： Akhtar RA,Abdel-Latif AA

更新日期：1986-01-01 00:00:00

abstract：:The metabolism of 2-fluoro-2-deoxy-D-glucose (FDG) in vivo was observed noninvasively in rat brain using 19F nuclear magnetic resonance (NMR) spectroscopy following an intravenous injection of FDG (400 mg/kg). At 3 h after infusion, four resonances with discrete chemical shifts were resolved. Chemical shift analysis o...

journal_title：Journal of neurochemistry

authors： Nakada T,Kwee IL,Conboy CB

更新日期：1986-01-01 00:00:00

abstract：:[3H]Cocaine dissociates from its binding sites in the mouse cerebral cortex with a half-time of 25 s. The dissociation kinetics in the striatum is consonant with the presence of two populations of sites with dissociation half times of 2 s and 27 s, comprising 88% and 12%, respectively, of the total binding sites. On t...

journal_title：Journal of neurochemistry

authors： Reith ME,Sershen H,Lajtha A

更新日期：1986-01-01 00:00:00

abstract：:1-[2-(4-Aminophenyl)ethyl]-4-(3-trifluoromethylphenyl)piperazine (PAPP) inhibits [3H]5-hydroxytryptamine (5-HT, serotonin) binding to 5-HT1A and 5-HT1B sites in rat brain with apparent equilibrium dissociation constants (KD) of 2.9 and 328 nM, respectively. [3H]PAPP was synthesized, its binding to central serotonin re...

journal_title：Journal of neurochemistry

authors： Ransom RW,Asarch KB,Shih JC

更新日期：1986-01-01 00:00:00

abstract：:Although 3,4-dihydroxyphenylethylamine (dopamine, DA) and vasoactive intestinal peptide (VIP) have been reported to stimulate adenylate cyclase activity in the rabbit retina, possible interactions between VIP-sensitive and DA-sensitive adenylate cyclase systems have not been previously investigated. To elucidate the i...

journal_title：Journal of neurochemistry

authors： Pachter JA,Lam DM

更新日期：1986-01-01 00:00:00

abstract：:In spite of biochemical and autoradiographic evidence for glucocorticoid binding sites in the spinal cord (SC), events occurring after the preliminary step of hormone binding were not studied. In this investigation, we have examined the transformation (activation) of the cytosolic receptor coupled to [3H]dexamethasone...

journal_title：Journal of neurochemistry

authors： Ortí E,Tornello S,De Nicola AF

更新日期：1985-12-01 00:00:00

abstract：:The ontogeny of muscarinic receptors was studied in human fetal striatum, brainstem, and cerebellum to investigate general principles of synaptogenesis as well as the physiological balance between various chemical synapses during development in a given region of the brain. [3H]Quinuclidinyl benzilate ([3H]QNB) binding...

journal_title：Journal of neurochemistry

authors： Ravikumar BV,Sastry PS

更新日期：1985-12-01 00:00:00

abstract：:Leptinotoxin-h (LPTx), a neurotoxin (otherwise designated beta-leptinotarsin-h) known to stimulate the release of neurotransmitters from synapses, was purified from the hemolymph of the potato beetle, Leptinotarsa haldemani, by a simplification of the procedure originally developed by Crosland et al. [Biochemistry 23,...

journal_title：Journal of neurochemistry

authors： Madeddu L,Pozzan T,Robello M,Rolandi R,Hsiao TH,Meldolesi J

更新日期：1985-12-01 00:00:00

abstract：:Aging was associated with an increase in the density of specific binding sites for [3H]imipramine in postmortem specimens of human hypothalamus, frontal cortex, and parietal cortex. In general, [3H]imipramine binding was not affected by factors considered difficult to control in postmortem studies, i.e., time from dea...

journal_title：Journal of neurochemistry

authors： Severson JA,Marcusson JO,Osterburg HH,Finch CE,Winblad B

更新日期：1985-11-01 00:00:00

abstract：:Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate-saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent Km of approximately 1 mM. Maintenance of the cells in medium containing thyroid-hormone-free serum for 3, 6, or 9...

journal_title：Journal of neurochemistry

authors： Roeder LM,Williams IB,Tildon JT

更新日期：1985-11-01 00:00:00

abstract：:We have recently isolated from bovine adrenal medulla a novel C-terminally amidated opioid peptide, amidorphin, which derives from proenkephalin A. Amidorphin revealed a widespread distribution in bovine, ovine, and porcine tissue. Particularly high concentrations of amidorphin immunoreactivity were detected in adrena...

journal_title：Journal of neurochemistry

authors： Liebisch DC,Seizinger BR,Michael G,Herz A

更新日期：1985-11-01 00:00:00

abstract：:L-Aspartate N-acetyltransferase, a nervous system enzyme that mediates the synthesis of N-acetyl-L-aspartic acid, has been characterized. In the presence of acetyl-CoA, L-aspartate was acetylated 10-fold more efficiently than L-glutamate, and the acetylation of aspartylglutamate was not detectable. Within the nervous ...

journal_title：Journal of neurochemistry

authors： Truckenmiller ME,Namboodiri MA,Brownstein MJ,Neale JH

更新日期：1985-11-01 00:00:00

abstract：:Capillaries in vertebrate brain have unique permeability properties that make up the blood-brain barrier (BBB). Although it is known that capillaries are innervated by nerve endings of intracerebral origin and that brain capillary function is likely acutely regulated by neuronal inputs, the possible mechanisms of neur...

journal_title：Journal of neurochemistry

authors： Pardridge WM,Yang J,Eisenberg J

更新日期：1985-10-01 00:00:00

abstract：:Fresh myelin, isolated from brainstems of adult rats, was incubated in the presence of Cu2+ and H2O2. Electrophoretic analysis of the reisolated myelin membrane revealed a gradual loss of the protein moiety from the characteristic pattern and an increase in aggregated material appearing at the origin of the gel. The a...

journal_title：Journal of neurochemistry

authors： Konat GW,Wiggins RC

更新日期：1985-10-01 00:00:00

abstract：:Previous work from this laboratory has shown that in PC12 cells the phosphorylation of a specific soluble protein is decreased by nerve growth factor treatment. The protein, designated Nsp100, and its kinase have been separated and partially purified from PC12 cells. In the present work, the tissue distribution of Nsp...

journal_title：Journal of neurochemistry

authors： Hama T,Guroff G

更新日期：1985-10-01 00:00:00

abstract：:Cricket (Acheta domesticus) terminal abdominal ganglia (TG) contain high concentrations (approximately 2 pmol/mg protein) of muscarinic and nicotinic cholinergic binding sites, based on the capacity of TG to bind specifically the labelled ligands L-[3H]quinuclidinyl benzilate ([3H]QNB) and [125I]alpha-bungarotoxin ([1...

journal_title：Journal of neurochemistry

authors： Meyer MR,Reddy GR

更新日期：1985-10-01 00:00:00

abstract：:Axonal transport of glycoconjugates was studied in the motoneurons of rat sciatic nerve following injection of [3H]glucosamine into the lumbosacral spinal cord. After varying time intervals, the sciatic nerve was exposed, and two ligatures were tied for collection of materials undergoing anterograde and retrograde tra...

journal_title：Journal of neurochemistry

authors： Aquino DA,Bisby MA,Ledeen RW

更新日期：1985-10-01 00:00:00

abstract：:An oligodendroglial specific property, glucocorticoid regulation of glycerol-3-phosphate dehydrogenase (GPDH) levels, was inhibited in C6 rat glioma cells when 4 beta-phorbol 12-myristate 13-acetate (PMA) was added to the cultures. PMA inhibited GPDH induction in both logarithmic- and stationary-phase cells. These eve...

journal_title：Journal of neurochemistry

authors： Bressler JP,Weingarten D,Kornblith PL

更新日期：1985-10-01 00:00:00

abstract：:When indole-3-acetaldehyde was incubated with rat brain tissue, an aldehyde dehydrogenase-independent irreversible disappearance of the aldehyde was found. This was accompanied by an increase in absorbance at 240-400 nm, with a peak at 310 nm. The results suggested that this change in absorbance was caused by a membra...

journal_title：Journal of neurochemistry

authors： Nilsson GE,Tottmar O

更新日期：1985-09-01 00:00:00

abstract：:Intraperitoneal injection of the cyclic imine 1-piperideine in mice resulted in measurable quantities of 5-aminopentanoic acid in brain. 5-Aminopentanoic acid is a methylene homologue of gamma-aminobutyric acid (GABA) that is a weak GABA agonist. 5-Aminopentanoic acid formed in the periphery was ruled out as the sourc...

journal_title：Journal of neurochemistry

authors： Callery PS,Geelhaar LA

更新日期：1985-09-01 00:00:00

abstract：:A procedure is described for the rapid preparation of nerve ending particles (synaptosomes) from 11 regions of one rat brain. The synaptosomal fractions have been characterized by electron microscopy and determination of four marker enzymes, i.e., glutamate decarboxylase (GAD), acetylcholinesterase, succinate dehydrog...

journal_title：Journal of neurochemistry

authors： Löscher W,Böhme G,Müller F,Pagliusi S

更新日期：1985-09-01 00:00:00

abstract：:In this study we examined the interaction of opiates with kappa binding sites in the bovine adrenal medulla. [3H]Ethylketocyclazocine (EKC), [3H]etorphine, and [3H]bremazocine stereoselective bindings were used to assay these interactions. The kappa sites were found to be heterogeneous: [3H]bremazocine identified with...

journal_title：Journal of neurochemistry

authors： Castanas E,Bourhim N,Giraud P,Boudouresque F,Cantau P,Oliver C

更新日期：1985-09-01 00:00:00

abstract：:Postmortem changes in catecholamines, indoleamines, and their metabolites in rat brain regions following decapitation were determined by LCEC. In the three regions studied neurotransmitter levels declined after decapitation, whereas the metabolite levels increased. Microwave irradiation at 10 kW rapidly inactivated br...

journal_title：Journal of neurochemistry

authors： Ikarashi Y,Sasahara T,Maruyama Y

更新日期：1985-09-01 00:00:00

abstract：:A mitochondrial fraction, purified from pig brain, was found to contain associated polypeptides with the same electrophoretic migration and isoelectric points as the alpha- and beta-tubulin subunits present in brain microtubules. When analyzed by Western blotting these polypeptides reacted specifically with purified t...

journal_title：Journal of neurochemistry

authors： Hargreaves AJ,Avila J

更新日期：1985-08-01 00:00:00

abstract：:The sites of interaction of quinacrine with synaptic membranes were labeled with quinacrine mustard. Quinacrine mustard had an inhibitory effect on depolarization-induced calcium uptake by synaptosomes similar to that of quinacrine. The inhibition of depolarization-induced calcium uptake by quinacrine was reduced by 7...

journal_title：Journal of neurochemistry

authors： Ohta A,Baba A,Iwata H

更新日期：1985-08-01 00:00:00

abstract：:Procedures are described for the solubilization of adenosine uptake sites in guinea pig and rat brain tissue. Using [3H]nitrobenzylthioinosine [( 3H]NBI) the solubilized site is characterized both kinetically and pharmacologically. The binding is dependent on protein concentration and is saturable, reversible, specifi...

journal_title：Journal of neurochemistry

authors： Verma A,Houston M,Marangos PJ

更新日期：1985-08-01 00:00:00

abstract：:Previous histological, electrophysiological, and biochemical reports have addressed the hypothesis that serotonin functions as a neurotransmitter in mammalian retinas. We have tested the effect on the levels of cyclic AMP of the application of exogenous serotonin, 5-methoxytryptamine, melatonin, and 5-methoxydimethyl-...

journal_title：Journal of neurochemistry

authors： Blazynski C,Ferrendelli JA,Cohen AI

更新日期：1985-08-01 00:00:00

abstract：:(-)-[3H]Nicotine was found to bind specifically to membranes of human brains obtained at autopsy. The binding was stereospecific, (-)-nicotine being 40 times more potent than (+)-nicotine in displacing labeled (-)-nicotine. Saturation binding studies revealed the presence of two binding sites with dissociation constan...

journal_title：Journal of neurochemistry

authors： Shimohama S,Taniguchi T,Fujiwara M,Kameyama M

更新日期：1985-08-01 00:00:00

abstract：:Adenylate cyclase in homogenates of Drosophila melanogaster is heterogeneous with respect to its affinity toward MgATP and its subcellular distribution. Km values for MgATP range, under similar assay conditions, from approximately 10(-5) M to approximately 10(-3) M, depending on the body region and on the subcellular ...

journal_title：Journal of neurochemistry

authors： Dudai Y,Zvi S

更新日期：1985-08-01 00:00:00

abstract：:Quinolinic acid (QUIN), an excitotoxic compound present in the mammalian CNS and periphery, has been hypothetically linked to human neurodegenerative disorders such as Huntington's disease and epilepsy. Quinolinic acid phosphoribosyltransferase (QPRT), the catabolic enzyme of QUIN, is found in the CNS and peripheral o...

journal_title：Journal of neurochemistry

authors： Foster AC,Schwarcz R

更新日期：1985-07-01 00:00:00

abstract：:The lipids of gastrocnemius muscle from normal and dystrophic (dy) mice of the Bar Harbor, 129Re strain were studied. Animals were fed diets containing either 3.1% or 1.1% of total calories as linoleic acid. Lipid analyses were also done on muscle from a new mouse mutant, A2G-adr, which has abnormal muscle function, c...

journal_title：Journal of neurochemistry

authors： Rebello T,Watts DC

更新日期：1985-07-01 00:00:00

abstract：:The present study demonstrates that [3H]imipramine binds to both high- and low-affinity imipramine binding components on membranes prepared from rat cerebral cortex. Scatchard and computer analyses of saturation experiments using a wide range of [3H]imipramine concentrations (0.5 nM-50 nM) revealed the presence of two...

journal_title：Journal of neurochemistry

authors： Conway PG,Brunswick DJ

更新日期：1985-07-01 00:00:00

abstract：:The changes in activity of phospholipid methyltransferase I and [3H]S-adenosyl-L-homocysteine ([3H]SAH) binding were determined in cortical membrane preparations from newborn rats and rats 1, 2, and 8 months old. The activity of phospholipid methyltransferase I and the [3H]SAH binding were significantly greater (respe...

journal_title：Journal of neurochemistry

authors： Gharib A,Rey C,Fonlupt P,Sarda N,Pacheco H

更新日期：1985-07-01 00:00:00

abstract：:The specific binding of [3H]8-hydroxy-2-(di-n-propylamino)tetralin ([ 3H]8-OH-DPAT) to 5-hydroxytryptamine (5-HT)-related sites was investigated in several regions of the rat brain. Marked differences were observed in the characteristics of binding to membranes from hippocampus, striatum, and cerebral cortex. Hippocam...

journal_title：Journal of neurochemistry

authors： Hall MD,el Mestikawy S,Emerit MB,Pichat L,Hamon M,Gozlan H

更新日期：1985-06-01 00:00:00